Sever Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2),the causative agent of the Coronavirus disease(Covid-19) pandemic, has so far resulted in more than 1.1M deaths and 40M cases worldwide with no confirmed remedy yet available. Since the frist outbreak in Wuhan, China in December 2019, researchers across the globe have been in a race to develop therapies and vaccines against the disease.SARS-CoV-2 ,similar to other previously identified Coronaviridae family members, encodes several structural proteins , such as spike , envelope , membrane ,and nucleocapsid, that are responsible for host penetration , binding ,recycling, and pathogensis. In the winter of 2020,many areas second burst, the medical testing institutions have a lot of testing pressure, If a crowd gathers, it is very susceptible as well. So to test by self in home will be Desperately .The SARS-CoV-2 Antigen Rapid Test Kit is a lateral flow chromatographic immunoassay for the qualitative detection of SARS-CoV-2 Antigen in human saliva specimens. Saliva test will be easier than swab test. People don’t need to collect the specimen in deep throat and Oropharynx. It will be more comfortable than swab test.

The SARS CoV-2 Antigen Rapid Test（Saliva Test） is a lateral flow chromatographic immunoassay. The test cassette consists of: 1)a burgundy colored conjugate pad containing Mouse anti-SARS-CoV-2 antibody conjugated with colloid gold (SARS CoV-2 conjugates) and mouse IgG-gold conjugates, 2) a nitrocellulose membrane strip containing two test bands (test band) and a control band (C band). The test band is pre-coated with Mouse anti-SARS-COV-2 antibody .The C band is pre-coated with goat anti mouse IgG.

When an adequate volume of test specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. SARS-CoV-2virus if present in the specimen will bind to the monoclonal mouse anti- SARS-CoV-2 antibody conjugates. The immunocomplex is then captured on the membrane by the pre-coated mouse anti- SARS-CoV-2 antibody, forming a burgundy colored T band, indicating a Covid-19 antigen positive test result. Absence of test band (T) suggests a negative result. The test contains an internal control (C band) which should exhibit a burgundy colored band of the immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate regardless of the color development on any of the test bands. Otherwise, the test result is invalid, and the specimen must be retested with another device.