Medical immune colloidal gold technology refers to a method of medical detection using colloidal gold as a marker and nitrocellulose membrane as a solid carrier according to the color characteristics of the detection line.
1 application of qualitative detection
The application of immune colloidal gold technology is more mature, and there are two main ways in qualitative detection: DIGFA (speckle immunogold percolation) method and GICA (colloidal gold immunochromatography) method. The above two methods are more mature in the field of medical examination, which is based on their simple operation, short testing time and high sensitivity. Medical on-site diagnosis has become the most common method.
（) DIGFA assay
In the initial screening test, DIGFA detection method is an effective measure, its application in symptom diagnosis is good, the effect is also affirmed, its application prospect is a good one. the catecholamine method is also a common detection method. in contrast, the digfa method is more effective in the clinical effect of fecal occult blood test, and its anti-interference ability and sensitivity are better than the catecholamine method. The researchers used DIGFA in the selection of serum syphilis antibody test measurement method, the specificity of this method is 100% and the forest sensitivity is 94%, which has the same excellent properties as enzyme-linked immunosorbent assay (elisa) and flow microspheres carrier technique (fma).
（2) GICA Detection Method
Colloidal gold immunochromatography (GICA), a new measure of immunochromatographic testing in the 1990s, was used to detect candida vaginalis with high specificity and sensitivity. GICA detection in the field Can be carried out quickly, the effect is good. The sensitivity of creatine kinase isoenzyme, troponin and myoglobin were 96.3%,85.2% and 100% in the diagnosis of acute myocardial infarction.
Application of quantitative and semi-quantitative detection
application atmosphere quantitative detection and semi-quantitative detection of the amount of immune colloidal gold. Red or pink are the most common colors of colloidal gold itself Color, through the optical sensor or detection line color depth of the naked eye to determine the sample quantitative and semi-quantitative judgment.
（) semi-quantitative detection
In the early stage of the study, some scholars suggested that in the color development of nitrocellulose membrane, the area of color development is proportional to the concentration of specific antibody. A relatively simple, easy-to-operate and efficient method for detecting neomycin residues in milk was constructed by using immunocolloid technology, and the results were accurate. There are also researchers A simple and rapid method for detecting trace proteins in human urine is constructed, which has an irreplaceable effect on the technology of immune fluid gold.
（2) Quantitative detection
The application of immunofluid gold technology is based on the principle of the relationship between the color depth of the nitrocellulose membrane detection line, the concentration of the object to be tested and the depth of the detection line. In quantitative detection, the reagent strips are first scanned with optical sensors, compared with the standard curve, and then the results of quantitative detection and analysis are obtained. Reagent strip detection line in the detection of color dynamic changes, this is It is shown that the different conditions of reagent strip manufacturing technology, the environment of the sample and the inspection environment will have a corresponding impact on the detection process and results, so how to minimize the impact or make the impact more stable has been an important research area of relevant researchers. For this reason, many researchers have made a lot of achievements, such as the T/C ratio method (detection line / quality control line), which is based on the above detection line of cellulose nitrate and quality control line color rendering factors are basically the same. Using the ratio of absorbance between T and C lines, we can ignore the influence of time, temperature, sample’s own difference and so on. make the detection results more persuasive, and this method is also a very persuasive detection method. In addition, some scholars have constructed an immune colloidal gold detection method, which can effectively detect Staphylococcus aureus enterotoxin B in the sample and quantitatively analyze its content, ranging from 8 ng/mL to 1000 ng/mL.
3 Other applications With the development of the technology of immune colloidal gold, it also has a very good performance in the application of immune electron microscope. In the research field, the relevant researchers have already effectively combined the immune colloidal gold technology with the electron microscope technology, and formed the immune colloidal gold electron microscope technology, which has the characteristics of high sensitivity, strong specificity and accurate positioning, and has been paid attention to in the field of electron microscope technology. Immune colloidal gold technology is also widely used in nano-magnetism and accounting aptamers. Nano-magnetism and accounting aptamers are specially designed to identify the target molecules from more complex biological systems The detection sensitivity and detection rate can be improved effectively by the concentration of target molecules. Immune fluid gold technology also has a prominent application in many joint detection techniques, and the multi-minded joint detection technology can simultaneously detect a variety of components, which plays an important role in the detection time and detection cost saving optimization.
4 knot Language
Researchers and relevant technicians should have an in-depth understanding and use of immune fluid gold technology, and combine with other methods to construct a new detection method, so that the detection technology can take a new stage order, so that immune fluid gold technology is more widely used in the field of medical testing, improve the efficiency and accuracy of the field of medical testing.
